High performance liquid chromatography of long-chain acylcarnitine and phospholipids in fatty acid turnover studies

High performance liquid chromatography of long-chain acylcarnitine and phospholipids in fatty acid turnover studies

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In this paper we describe a rapid, isocratic high performance liquid chromatography (HPLC) method for the study of radioactive fatty acid incorporation into complex lipids of human erythrocytes, which allows the simultaneous separation of the major phospholipid classes and long-chain acylcarnitines.The lipid extract of erythrocytes pulsed with radioactive fatty acids was injected into an HPLC system equipped iphone 13 dallas with a silica column.The individual components eluted were monitored by ultraviolet absorption and radioactive emission.With respect to the UV profile, the radioactive profile showed an additional peak between phosphatidyl-choline and phosphatidylethanolamine, which was identified as long-chain acylcarnitine by different experimental approaches.

The radioactivity recovered in the long-chain acylcarnitines contains essential information enabling telemarkskongen flue definition of acyl trafficking in red cells.